RealCycler

Detection of pathogens through Real-time PCR

Infectious meningoencephalitis

Respiratory tract infections

Gastrointestinal infections

Bacterial resistances

STDs

Opportunistic infections

Systemic infections 

Congenital and perinatal infections

Infectious meningoencephalitis

Meningoencephalitis are characterized by an inflammation of the brain and of the meninges. When it is due to an infection, its origin can be viral or bacterial.

These infections are especially dangerous for children, having a higher mortality rate among them than among adults. The early detection of the pathogen is vital to provide a correct treatment and minimize the associated risk.

06/2022

Artícle in "Journal of Medical Virology"

02/2021
Scientific letter in the "Enfermedades Infecciosas y Microbiología Clínica" journal 

10/2019
Article in "Japanese Journal of Infectious Diseases"

12/2018
Article in Anales de Pediatría magazine

10/2018
Article in Pediatría Integral magazine

05/2018
Abstract in XXII Congreso de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica, 24-26 Mayo 2018.

05/2018
Abstract in XXII Congreso de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica, 24-26 Mayo 2018.

05/2018
Abstract in XXII Congreso de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica, 24-26 Mayo 2018.

11/2017
Poster at the XVLI Congresso Nazionale Associazione Microbiologi Clinici Italiani, 11-14 noviembre 2017 Rimini-Palacongressi

05/2017
Poster at the XXI Congress of the Spanish Society of Infectious Diseases and Clinical Microbiology, 11-13 May 2017.

05/2017
Poster at the XXI Congress of the Spanish Society of Infectious Diseases and Clinical Microbiology, May 11-13, 2017.

05/2017
Poster at the XXI Congress of Spanish Society of Infectious Diseases and Clinical Microbiology, 11-13 May 2017.

02/2017
Article in the journal Infectious Diseases and Clinical Microbiology.

11/2015
Doctoral thesis at the Complutense University of Madrid.

07/2015
Article in the journal of the Spanish Society of Infectious Diseases and Clinical Microbiology.

02/2014
Article in the journal of the Spanish Society of Infectious Diseases and Clinical Microbiology.

Respiratory tract infections

Respiratory tract infections can affect both upper and lower airways. The latter are less frequent but more serious. They can be caused by viruses, bacteria or fungi, although most are of viral origin. In some cases they can trigger serious clinical conditions, such as pneumonia. Identifying the causative agent of the disease is essential to determine the severity of the disease and choose an appropriate treatment.

10/2020

Article in Anales de Pediatría journal

“Epidemiological and clinical analysis of comunity-acquired Mycoplasma pneumonia in children from a Spanish population, 2010-2015”


01/2020

Letter to the Editor in the Journal of Infection magazine

“Clinical significance of Pneumocystis jirovecii DNA detection by real-time PCR in hematological patient respiratory specimens”


01/2020

Article in "Molecular Therapy"

"First-in-Human, First-in-Child Trial of Autologous MSCs Carrying the Oncolytic Virus Icovir-5 in Patients with Advanced Tumors"


07/2019

Article in Journal of Surgery and Medicine

“Clinical and demographic characteristics of influenza B outbreak in Erzincan province of Turkey”


11/2018

Poster at the XLVII Congresso Nazionale Associazione Microbiologi Clinici Italiani, 10-13 noviembre 2018 Rimini-Palacongressi

“Real-time PCR nella ricerca di Pneumocystis jirovecii: valutazione di un kit commerciale e suo impiego all’interno di un algoritmo diagnostico”


11/2017

Poster at the XVLI Congresso Nazionale Associazione Microbiologi Clinici Italiani, 11-14 noviembre 2017 Rimini-Palacongressi

"Valutazione della presenza di Pneumocystis jirovecii in campioni clinici provenienti da pazienti con sospetta infezione polmonare. Confronto di due metodiche diagnostiche: immunofluorescenza e real-time PCR."


09/2017

Scientific Letter in the journa Archivos de Bronconeumología

“Micobacteriosis pulmonar en un paciente en tratamiento crónico con metotrexato” 


05/2017

Poster at the XXI Congress of the Spanish Society of Infectious Diseases and Clinical Microbiology, 11-13 May 2017.

“Diagnóstico de la infección por Pneumocystis jirovecii en pacientes no VIH mediante PCR cuantitativa”


05/2017

Article in the Anales del Sistema Sanitario de Navarra (Navarra Health System Annals)

“Comparación de las características clínicas y diagnósticas de los subgrupos A y B del virus respiratorio sincitial”


01/2017

Article in Diagnostic Microbiology and Infectious Disease Journal

"Comparison of a commercial real-time PCR assay, RealCycler® PJIR kit, progenie molecular, to an in-house real-time PCR assay for the diagnosis of Pneumocystis jirovecii infections"


11/2016

Poster at the Congress of the Association of Italian Clinical Microbiologists (AMCLI), Italy.

"Ricerca di Pneumocystis jirovecii: Real time PCR vs immunofluorescenza"


05/2016

Poster at the XX Congreso Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (Barcelona, Spain)

"Comportamiento epidemiológico de Mycoplasma pneumoniae en el periodo 2008-2015"


05/2016

Poster at the XX Congreso Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (Barcelona, Spain)

"Aportación de las técnicas moleculares en el diagnóstico microbiológico de la neumonía por Mycoplasma pneumoniae"


03/2016

Poster at the Congress of Société Française de Mycologie Médicale (SFMM), France

"Comparison of a new commercial real-time PCR assay (RealCycler PJIR kit, Progenie Molecular) to an in-house real-time PCR assay for the diagnosis of Pneumocystis jirovecii infections"


(Comparación de un nuevo test comercial por PCR a tiempo real (kit RealCycler PJIR, Progenie Molecular) frente a un test in-house por PCR a tiempo real para el diagnóstico de infecciones por Pneumocystis jirovecii)


07/2015

Article in the journal of the Spanish Society of Infectious Diseases and Clinical Microbiology.

"Análisis de resultados del Programa Externo de Control de Calidad SEIMC. Año 2013"


05/2015

Poster at the XIX Spanish Society of Infectious Diseases and Clinical Microbiology Congress (Seville)

"Evaluación del sistema multianalítico mariPOC® en la determinación de Virus Respiratorio Sincitial e Influenza A/B en población pediátrica"


04/2015

Póster en el 25º Congreso Europeo de Microbiología Clínica y Enfermedades Infecciosas (ESCMID), Copenhague

"Comparison of two commercial real-time PCR assays for influenza virus detection: IMDxTM Flu A/B and RSV for Abbott m2000TM and Real Cycler® FLURSV"


02/2014

Article in the journal of the Spanish Society of Infectious Diseases and Clinical Microbiology.

"Análisis de resultados del Programa de Control de Calidad Externo SEIMC. Año 2012"


05/2013

Poster at the XVII Spanish Society of Infectious Diseases and Clinical Microbiology (Zaragoza, Spain)

"Espectro de virus respiratorios en niños con infección de vías respiratorias inferiores"


04/2013

Abstract of the XVI ECCMID Congress

"Nosocomial outbreak of Klebsiella pneumoniae producing extended-spectrum beta-lactamase and OXA48 at Carlos Haya Regional Hospital in Málaga (Spain)"


Gastrointestinal infections

Gastrointestinal infections cause gastroenteritis and mainly affect the stomach, small intestine and colon. The causal agents of these infections can be viruses, bacteria or parasites.

Gastrointestinal infections are one of the most frequent infectious diseases and one of the most important causes of morbidity and mortality in children and the elderly. In this context, a fast diagnosis is key to offer an adequate treatment.

11/2019

Article in "The Open Microbiology Journal"

"Analysis of Routine and Integrative Data from Clostridioides difficile Infection Diagnosis and the Consequent Observations"


04/2019

Comunication in Clínica Española magazine

"I-039 - Toxina directa en heces, toxina binaria y diarrea por clostridium difficile"


05/2018

Abstract in the XXII Congreso de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica, 24-26 Mayo 2018.

“Valor diagnóstico de la serología en un brote de parotiditis”


05/2018

Abstract in the XXII Congreso de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica, 24-26 Mayo 2018.

“Utilidad de la PCR a tiempo real en el diagnóstico de la neumonía causada por Pneumocystis jirovecii” 


07/2015

Article in the journal of the Spanish Society of Infectious Diseases and Clinical Microbiology.

"Análisis de resultados del Programa Externo de Control de Calidad SEIMC. Año 2013"


02/2014

Article in the journal of the Spanish Society of Infectious Diseases and Clinical Microbiology.

"Análisis de resultados del Programa de Control de Calidad Externo SEIMC. Año 2012"


05/2013

Poster at the XVII Congreso Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (Zaragoza, Spain)

"Caracterización fenotípica de aislados de Pseudomonas aeruginosa productores de carbapenemasas y detección de genes de resistencia tipo VIM"


05/2013

Poster at the XVII Congress Spanish Society of Infectious Diseases and Clinical Microbiology (Zaragoza, Spain)

"Evaluación de Realcycler OXVI® para la detección de carbapenemasas OXA-48 y VIM"


Bacterial resistances

Bacterial resistances are based on molecular mechanisms that allow bacteria to survive antibiotics. They are increasing alarmingly, they are associated with higher mortality rates and due to them the cost of treatments is much higher. The identification of the type of resistance that a bacterium presents is essential to provide an adequate antibiotic treatment to the infection, increase the probabilities of recovery and reduce hospital costs.

07/2019

Article in Infection and Drug Resistance magazine.

“Molecular and phenotypical characterization of two cases of antibiotic-driven ceftazidimeavibactam resistance in blaKPC-3-harboring Klebsiella pneumoniae”


07/2019

Article in Official Journal of the Spanish Society of Chemotherapy

“Evaluación de una prueba rápida para la detección de PBP2a en Staphylococcus aureus”


02/2019

Article in Enfermedades Infecciosas y Microbiología Clínica magazine

"Caso importado de infección por Shigella sonnei portadora de betalactamasa de espectro extendido CTX-M-15"


05/2018

Abstract in the XXII Congreso de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica, 24-26 May 2018.

“Carbapenemasas en aislados de Pseudomonas putida en el norte de España”


05/2018

Abstract in the XXII Congreso de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica, 24-26 May 2018.

“Evaluación del sistema automatizado HB&L carbapenemase para la detección de portadores rectales de enterobacterias productoras de carbapenemasas en un hospital de tercer nivel”


04/2018

Abstract in the 28º Congreso Europeo de Microbiología Clínica y Enfermedades Infecciosas

“A practical diagnostic algorithm for screening and confirmation of carbapenemase production in Enterobacteriaceae in an OXA-48 endemic region using temocillin and ertapenem discs”


03/2018

Article in the Journal of Infection and Public Health magazine

"Remitting infections due to community-acquired Panton–Valentine leukocidin-producing Staphylococcus aureus in the Milan area"


11/2017

Article in International Journal of One Health

“Mingling of human and veterinary strains of Staphylococcus aureus: An emerging issue in health-care systems”


11/2017

Poster in the XVLI Congresso Nazionale Associazione Microbiologi Clinici Italiani, 11-14 noviembre 2017 Rimini-Palacongressi

"Miosite e osteomielite da mrsa pvl+ con quadro settico in paziente di ta’ pediatrica"


10/2017

Poster at the meeting of the "Sociedad Andaluza de Microbiología y Parasitología Clínica (SAMPAC)"

"Evaluación del kit RealCycler REZERO‐U/ REZERO‐G en pacientes de UCI de un hospital de tercer nivel" 


05/2017

Poster at the XXI Congress of the Spanish Society of Infectious Diseases and Clinical Microbiology, 11-13 May 2017.

“Klebsiella pneumoniae co-productora de carbapenemasa tipo NDM y OXA-48: 5 casos en un hospital terciario”


11/2016

Poster at the Association of Clinical Italian Microbiologists Congress (AMCLI), Italy

"Caratterizzazione dei meccanismi di resistenza e tipizzazione di Citrobacter spp multi-antibioticoresistente (MDR)"


11/2016

Poster at the Association of Clinical Italian Microbiologists Congress (AMCLI), Italy

"Circolazione di ceppi di S. aureus di provenienza umana e animale: un problema emergente in ambito sanitario"


11/2016

Poster at the Association of Clinical Italian Microbiologists Congress (AMCLI), Itay

"Ridotta sensibilita’ al linezolid in ceppi di S. aureus Meticillino-Resistenti (MRSA) isolati in pazienti con Fibrosi Cistica (FC)"


04/2016

Poster at the 26th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), Amsterdam

"S. aureus with Panton-Valentine leukocidin gene among two related families in Milan"


04/2016

Poster at the 26th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), Amsterdam

"Epidemiology of Staphylococcus aureus strains Methicillin-Resistant and sensitive isolated in Cardiosurgical Area of the italian L. Sacco Hospital"óster en el 26th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID), Amsterdam


11/2015

Procedures in Clinical Microbiology. Recommendations of the Spanish Society of Infectious Diseases and Clinical Microbiology.

“Métodos microbiológicos para la vigilancia del estado de portador de bacterias multirresistentes”


10/2015

Poster at the Association of Clinical Italian Microbiologists Congress (AMCLI), Italy

 "Applicabilità della rilevazione dei meccanismi di resistenza con metodiche molecolari nell'Infection Control: descrizione di un'esperienza"


(Applicability of the detection of resistance mechanisms with molecular methods in Infection Control: a case report)


10/2015

Poster at the Association of Clinical Italian Microbiologists Congress (AMCLI), Italy

"Identificazione e ricerca di geni di resistenza in batteri Gram negativi da emocoltura"

(Identificación e investigación de genes de resistencia en bacterias Gram negativas en hemocultivo)


07/2015

Article in the journal of the Spanish Society of Infectious Diseases and Clinical Microbiology.

"Caracterización de mecanismos de resistencia por biología molecular: Staphylococcus aureus resistente a meticilina, ß-lactamasas de espectro extendido y carbapenemasas"


07/2015

Article in the journal of the Spanish Society of Infectious Diseases and Clinical Microbiology.

"Análisis de resultados del Programa Externo de Control de Calidad SEIMC. Año 2013"


05/2015

Poster at the XIX Spanish Society of Infectious Diseases and Clinical Microbiology Congress (Seville)

"Estudio de un algoritmo de diagnóstico precoz de bacteremia producida por bacilos gramnegativos"


05/2014

Poster at the 24th European Congress of Clinical Microbiology and Infectious Diseases (ESCMID), Barcelona

"Improved Laboratory Reporting Times And Clinical Impact For Panton-Valentine Leukocidin Testing Using The Progenie Realcycler SAPV Real-time P.C.R. Assay"


05/2014

Poster at the 24th European Congress of Clinical Microbiology and Infectious Diseases (ESCMID), Barcelona

"Cross-sectional prevalence study of CTX-M-type extended-spectrum beta-lactamases in Escherichia coli strains isolated in healthy poultry"


04/2014

Poster at the XVIII Congress Spanish Society of Infectious Diseases and Clinical Microbiology (Valencia, Spain)

"Descripción de la primera cepa clínica de Aeromonas hydrophila productora de metalo-ß-lactamasa VIM en España"


09/2013

Poster at the Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC) 2013 en Denver (Colorado)

"Real-time PCR Assays for the Detection of Carbapenemase-Producing Enterobacteriaceae (CPE) from Stool Samples"


11/2012

Oral communication at the XXV meeting of the Andalusian Society of Clinical Microbiology and Parasitology (Almeria).

"Detección de Carbapenemasa OXA-48 por técnicas moleculares en el contexto de un brote de Klebsiella pneumoniae"


Article in the journal Epidemiology & Infection

"Prevalence of Trypanosoma cruzi infection in pregnant Latin American women and congenital transmission rate in a non-endemic area: the experience of the Valencian Health Programme (Spain)"


Sexually transmitted diseases

Sexually transmitted diseases (STDs) are caused by bacteria, viruses or fungi, the most common being chlamydia, gonorrhea, syphilis and trichomoniasis. Every day, more than 1 million people contract an STD in the world, and the consequences appear on a sexual and reproductive level, as well as systemically. An early diagnosis using molecular techniques is an essential tool for the effectiveness of the treatment.

10/2020

Article in Tropical Medicine and Infectious Disease magazine

“Multiplex Recombinase Polymerase Amplification Assay for Simultaneous Detection of Treponema pallidum and Haemophilus Ducreyi in Yaws-like Lesions”


08/2020

Article in Clinical Microbiology and Infection magazine

“Evaluation of four commercial real-time PCR assays for the detection of lymphogranuloma venereum in Chlamydia trachomatis-positive anorectal samples”


05/2019

Article in the Sexually Transmitted Infections magazine

“Lymphogranuloma venereum presenting as an ulcer on the tongue”


07/2018

Article in the Enfermedades Infecciosas y Microbiología Clínica magazine

"Relevancia del tratamiento de portadores rectales asintomáticos de Mycoplasma genitalium en hombres que tienen sexo con hombres".


05/2018

Abstract at the XXII Congreso de la Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica, 24-26 Mayo 2018.

“Diagnóstico de sífilis primaria por PCR a tiempo real vs. serología” 


05/2016

Poster at the XX Congreso Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (Barcelona, Spain)

"Brote de linfogranuloma venéreo en Vizcaya (2014-2015)"


05/2016

Poster at the XX Congreso Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (Barcelona, Spain)

"Diagnóstico de linfogranuloma venéreo en células Vero y A-549"


02/2016

Article in the journal Actas Dermo-Sifiliográficas

"Sexually Transmitted Infections: Experience in a Multidisciplinary Clinic in a Tertiary Hospital (2010-2013)"


09/2015

Poster at the 29th IUSTI European Conference on Sexually Transmitted Infections (Barcelona)

"Real-time PCR and serology for the diagnosis of primary syphilis"


06/2015

Poster at the Journées Nationales d'Infectiologie (France)

"Ulcération syphilitique: Abandonnez le fond noir, passez à la PCR !"

(Ulceración sifilítica: Abandone el fondo negro y pase a la PCR !)


05/2015

Poster at the XIX Spanish Society of Infectious Diseases and Clinical Microbiology Congress (Seville)

"Linfogranuloma venéreo en el área sur de Gran Canaria (2012-2015)"


05/2015

Poster at the XIX Spanish Society of Infectious Diseases and Clinical Microbiology Congress (Seville)

"Utilidad de una PCR a tiempo real para Treponema pallidum en el diagnóstico de la sífilis primaria"


05/2014

Poster at the XXVII National Congress of the Spanish Association of Laboratory Technicians

"PCR a tiempo real para Treponema pallidum, Chlamydia trachomatis L1-L2-L3 y Haemophilus ducreyi causantes de úlceras genitales y rectales"


04/2014

Poster at the XVIII Congreso Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (Valencia, Spain)

"Diagnóstico de sífilis: serología y técnicas moleculares"


12/2013

Oral communication at the Catalan Society of Infectious Diseases and Clinical Microbiology.

"Taula Rodona: Sífilis 2013 Les proves"


Opportunistic and nosocomial infections

Nosocomial infections (hospital-acquired infections) can cause serious complications in hospitalized patients. Immunosuppressed patients are more susceptible to these infections, and in many cases it causes a deterioration of their condition. The fast detection of this diseases is an essential tool for its control and treatment.

05/2016

Poster at the XX Congreso Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (Barcelona, Spain)

"Infección por el virus BK en trasplantados renales en los últimos cinco años en el CHU A Coruña"


Systemic infections

Systemic infections are those that affect almost the entire body. Any localized infection, whatever its origin (respiratory, genitourinary, digestive, etc.) can potentially become systemic.

Many viruses frequently cause systemic infections. However, the most serious infections are those caused by bacteria (bacteremia) as they are frequently associated with sepsis.

Congenital and perinatal infections

Congenital or perinatal infections affect the fetus or the newborn, and may cause their death or serious sequels. During pregnancy, both maternal and fetal infections should be identified, analysing amniotic fluid, chorionic villus or even fetal blood for the latter.

The high mortality associated with these infections makes prevention, diagnosis, follow-up and treatment very important.