RealType

Detection of mutations in human DNA by Real-time PCR

RealType® kits allow the simultaneous detection of mutations and variants in human DNA. They are based on Real-time PCR. Just add the DNA from the sample and put it into the thermocycler.

RealType kits are the ideal products to use as a rapid and specific genotyping test. They analyse mutations and variants frequently related to different pathologies or with the response to a treatment.

They are recommended as first method of genetic study, given that they allow the direct diagnosis of a great number of patients in a fast and economic manner. They are also useful for the screening of asymptomatic population.
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  • Ready to use without pre-mixes
  • High capacity for allelic discrimination
  • Compatible with ABI 7500, Bio-Rad CFX96 and Mic qPCR
  • Compatible with different extraction systems
  • Fast results (2 hours approximately)
  • In vitro diagnostics CE-IVD marked
  • Supporting software: BioVisor RT

BioVisor© RT

Support software for RealType products

BioVisor RT software facilitates the interpretation of the results obtained with RealType products for the study of point mutations through genotyping tests by Real-time PCR.

BioVisor RT imports the fluorescence data from the PCR equipment in real time, analyzes them automatically and issues a report with the result.

The application is validated for the CFX96 (Bio-Rad), ABI7500 (Life Technologies) and MIC (Biomolecular Systems) instruments.

  • Users and permissions management
  • Automatic genotype determination
  • Data import from the PCR instrument
  • Interpretation of the results of each sample
  • Interpretation of the series according to the result of the controls
  • Issue of a results report
  • Compatible with the most used Real-time PCR instrument
  • File of the work series

RealType catalogue

AOC1 gene: rs10156191, rs1049742, rs1049793 and rs2052129

Ref.: DDAO-20, DDAO-50


APOE (Ɛ2, Ɛ3 and Ɛ4) gene: Cys112Arg and Arg158Cys

Ref.: APOE-10, APOE-20, APOE-50


Factor II: G20210A (Prothrombin gene)

Ref.: PTRO-10, PTRO-20, PTRO-50


Factor V gene: Arg506Gln

Ref.: FVLD-10, FVLD-20, FVLD-50


Factor V and Factor II genes: trombophilia Arg506Gln (FV Leiden) and G20210A (Prothrombin gene)

Ref.: FVPT-10, FVPT-20, FVPT-50


HFE gene: Hemochromatosis Cys282Tyr, His63Asp and Ser65Cys

Ref.: HECR-10, HECR-20, HECR-50


IL28B gene: rs12979860 and rs8099917

Ref.: IL28-10, IL28-20, IL28-50


MCM6 gene: 13910*T and 22018*A

Ref.: LACT-20, LACT-50


MTHFR gene: A1298C and C677T

Ref.: MTHF-10, MTHF-20, MTHF-50


SERPINA-1 gene: Glu342Lys, Val213Ala and Glu264Val

Ref.: AIAT-10, AIAT-20, AIAT-50