RealType® kits allow the simultaneous detection of mutations and variants in human DNA. They are based on Real-time PCR. Just add the DNA from the sample and put it into the thermocycler.
RealType kits are the ideal products to use as a rapid and specific genotyping test. They analyse mutations and variants frequently related to different pathologies or with the response to a treatment.
They are recommended as first method of genetic study, given that they allow the direct diagnosis of a great number of patients in a fast and economic manner. They are also useful for the screening of asymptomatic population.
BioVisor RT software facilitates the interpretation of the results obtained with RealType products for the study of point mutations through genotyping tests by Real-time PCR.
BioVisor RT imports the fluorescence data from the PCR equipment in real time, analyzes them automatically and issues a report with the result.
The application is validated for the CFX96 (Bio-Rad), ABI7500 (Life Technologies) and MIC (Biomolecular Systems) instruments.
SERPINA-1 gene: Glu342Lys, Val213Ala and Glu264Val
Ref.: AIAT-10, AIAT-20, AIAT-50
APOE (Ɛ2, Ɛ3 and Ɛ4) gene: Cys112Arg and Arg158Cys
Ref.: APOE-10, APOE-20, APOE-50
Factor V and Factor II genes: trombophilia Arg506Gln (FV Leiden) and G20210A (Prothrombin gene)
Ref.: FVPT-10, FVPT-20, FVPT-50
HFE gene: Hemochromatosis Cys282Tyr, His63Asp and Ser65Cys
Ref.: HECR-10, HECR-20, HECR-50
MTHFR gene: A1298C and C677T
Ref.: MTHF-10, MTHF-20, MTHF-50
IL28B gene: rs12979860 and rs8099917
Ref.: IL28-10, IL28-20, IL28-50