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RealCycler Compatibility

DNA/RNA extraction systems compatibility

Validated equipments

RealCycler Universal, and RealCycler Chic-Out kits have been validated for MagCore (RBC Bioscience), QiaCube (Qiagen), Arrow/Liaison IXT (DiaSorin) and Maxwell 16 (Promega). This means that the compatibility with these equipments has been checked through the performance of laboratory assays. 


MagCore

RBCBioscience


QiaCube

Qiagen

Arrow/Liaison IXT

DiaSorin

Maxwell

Promega

RealTime PCR instruments compatibility

Compatible equipments

RealCycler Monotest, RealCycler Universal and RealCycler Chic-Out kits are compatible with all DNA/RNA extraction systems available in the market, without the need to make any changes to the original protocols provided by the producer.

This is due to the  CHIC Technology (Competitive Heterologous Internal Control), on which the internal control system of amplification is based. This is our own developped technology and it allows the use of any extraction system without losing sensibility and it monitors sample's inhibition with high accuracy at the same time.                              

Even though the CHIC technology enables the monitoring of extraction process' quality, it's necessary to verify that its purification equipment or its manual extraction system let the user obtain DNA and RNA with the proper quality and quantity. It's recommended to use external quality controls (QCMD, NIBSC, etc) in order to conduct a self-validation of its extraction system. 

Validated equipments

RealCycler Universal, and RealCycler Chic-Out kits have been validated for SmartCycler (Cepheid), CFX96 (Bio-Rad), ABI 7500 (Life Techonologies), Rotor-Gene Q (Qiagen) and MIC (Biomolecular Systems). This means that the compatibility with these equipments has been checked, laboratory assays have been carried out to establish the proper parameters for the interpretation of results and that these equipments have been inlcuded in the Visor RealCycler software. 

Compatible equipments

RealCycler Universal, y RealCycler Chic-Out kits are compatible with the main real time PCR equipments. The following grid shows the fluorophores used for our products and the corresponding wave's longitude. You can check the compatibility of your equipment with the fluorophores used.